Last Monday (January 22, 2024), I collected water samples for LC-MS lab tests as part of my PFAS detection method research. Two (drinking water) of my five samples are to be processed through EPA Method 537.
Today, having performed multiple sample collections, I would like to walk my readers through the process of collecting water samples for EPA Method 537 with photographs.
Before collecting any samples, please make sure you have the following from the laboratory analyzing your samples:
- Two sealed HDPE PFAS sample containers with TRIZMA preservative inside. Some laboratories may have fewer bottles, and may provide two more bottles. I will explain this in the procedure down below.
- Chain-of-custody form
- Powder-free nitrile gloves
- A cooler filled with ice
With these items gathered, we can now start preparing to take our sample.
Make sure:
- You are wearing powderless nitrile gloves
- Do NOT use latex or vinyl gloves
- You have not handled any aerosolized perfumes, detergents, or insect repellents before sampling
- You have not touched any food packaging before sampling
Time to start the procedure!
- Put gloves on. Label your sample bottles with your initials, time and date of collection, and sample name.
- Run your cold tap for three minutes.
- After three minutes, narrow the water flow down until you can see through it.
- Open sample bottle and fill to its neck.
- Close the cap, and shake a couple times to mix the preservative.
- Depending on the laboratory, you may have more samples bottles to fill. Additionally, some laboratories may provide you a bottle filled with laboratory water and an empty bottle for the purpose of collecting a control sample. If you have extra sample bottles, repeat steps 4 and 5. If you have a laboratory water bottle, simply pour the water into the empty bottle and discard the now-empty bottle.
- Place your sample bottles and control (if applicable) into your cooler.
- Return your samples to the lab and you’re done!
I hope that this guide was helpful and instructive. If you have any questions, don’t hesitate to contact me at tomtomboy2011@gmail.com.
For more detailed instructions, visit this website.
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